USE OF STRAIN-SPECIFIC MONOCLONAL ANTIBODIES TO QUANTIFY WITHIN-FIELD TEMPORAL AND SPATIAL SPREAD OF SOYBEAN MOSAIC VIRUS IN SOYBEAN

USE OF STRAIN-SPECIFIC MONOCLONAL ANTIBODIES TO QUANTIFY WITHIN-FIELD TEMPORAL AND SPATIAL SPREAD OF SOYBEAN MOSAIC VIRUS IN SOYBEAN

Forrest W. Nutter, Jr., Patricia M. Schultz, and John H. Hill

Associate Professor, former Research Associate, and Professor; respectively,

Department of Plant Pathology, Iowa State University, Ames, Iowa 50011.

Strain-specific monoclonal antibodies were used to follow the temporal and spatial spread of soybean mosaic virus (SMV) . The technique allowed discrimination of the within-field spread of the released SMV strain from other SMV strains that periodically entered the field from exogenous sources and which have potential for affecting the model that best describes within-field disease progress. Analyses of the epidemic showed a logistic model best described the temporal spread of SMV in plots during 1991 to 1994. Using ordinary runs analysis, virus spread was generally random in 1991 and 1994 but was aggregated in 1992 and 1993.

Key words: risk assessment, virus epidemiology

Research was supported by State and Hatch Funds allocated to the Iowa Agriculture and Home Economics Experiment Station and, in part, by the Iowa Soybean Promotion Board, Pioneer Hi-Bred Internatiorial, Inc., and the Iowa Center for Advanced Technology Development.