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Proceedings and Papers
from the 1996 Risk Assessment Research Symposium


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Preface: Risk Assessment at the Crossroads
Chapter 1: Environmental Effects
Chapter 2: Bioremediation
Chapter 3: Field/Risk Assessment (Microorganisms)
Chapter 4: Gene Transfer (Plants)
Chapter 5: Gene Stability (Plants)
Chapter 6: Field/Risk Assessment (Plants)
Chapter 7: Gene Transfer and Stability (Microorganisms)
Chapter 8: Risk Assessment: (Microorganisms)
Chapter 9: Monitoring
Chapter 10: Fish
Chapter 11: Posters
Appendix A: Agenda of 1996 Symposium

Preface: Risk Assessment at the Crossroads

Risk Assessment At The Crossroads
M.A. Levin, J.S. Angle, T. McIntyre, and H. Yu

Chapter 1: Environmental Effects

Method Development To Assess Microbial Diversity In Soil
James Borneman, Sandra Austin-Phillips and Eric W. Triplett

Risk Of Gene Flow From Sorghum To "Johnsongrass"
Andrew H. Paterson and J. Mike Chandler

Managing Insect Resistance On Transgenic Crops: Model Predictions And Preliminary Experimental Results
Juliette Winterer and Joy Bergelson

Environmental Fate And Risk Assessment Of A Novel Forest-Weed Biocontrol Agent
William Hintz and Simon Shamoun

Acute Toxicity And Aqueous Solubility Of Some Condensed Thiopenes And Their Microbial Metabolites
Phillip M. Fedorak, David T. Seymour, Allen G. Verbeek, and Steve E. Hrudey

An Approach To Risk Assessment Based On Molecular Diversity Of Procaryotes From Soil And The Rhizosphere
Scott B. Bintrim, Ranjit Dasgupta, Timothy J. Donohue, Robert M. Goodman, Jo Handelsman, Deborah Joseph, and Gary P. Roberts

Chapter 2: Bioremediation

The Distribution Of Natural Chlorobenzoate Catabolic Genotypes At The Hyde Park Industrial Landfill Site
M.C. Peel and R.C. Wyndham

IS1071 As An Indicator Of Bacterial Adaptation To Inorganic And Organic Pollutants In Freshwater Environments
R.C. Wyndham, C.H. Nakatsu and M.C. Peel

Monitoring Potential Bioremediation Agents Using Reporter Gene-Marked Indigenous Bacterial Model
Charles W. Greer, Judith G. Godbout, Anca Mihoc, Claude Masson, and Maurice V. Cattaneo

Biotechnological Potential Of Heavy Water And Deuterated Compounds
D.J. Kushner, Alison Baker and T.G. Dunstall

Chapter 3: Field/Risk Assessment (Microorganisms)

Risk Assessment Of An Intergeneric Microorganism Involved In Bioremediation
Philip Sayre

Environmental Assessment Of Genetically Engineered Insect Viruses In Canadian Forestry
K.N. Barber, W.J. Kaupp, D.P. Kreutzweiser, L.S. England, A.S.D. Pang, S.R. Palli, and S.B. Holmes

Development Of Analytical Tools For Risk Assessment Of Genetically Modified Organisms
Mark Segal and Lois Blaine

Persistence Of Recombinant DNA In Soil
Laura S. England, Hung Loc, Jack T. Trevors, and Stephen Holmes

An Experimental Evaluation Of Raccoons As A Reservoir For Transmission Of Genetically Engineered Pseudorabies Virus Vaccine And Wildtype-Vaccine Recombinants
Ronald M. Weigel, Edwin C. Hahn, and Gail Scherba

Risk Assessment Of A Vaccinia Virus Recombinant Vaccine For Rinderpest
Tilahun Yilma, Leslie Jones, Fikre Yoseph, and Mebratu Gebre Yesus

Risk Assessment Of Recombination And Transcapsidation Of Tobacco Mosaic Virus With The Cucumber Mosaic Virus Coat Protein
Peter Palukaitis and Lee Zhang

Incidence Of Barley Yellow Dwarf Virus In Wild Grass Populations: Implications For Biotechnology Risk Assessment
Alison G. Power and Susanna K. Remold

In Situ Intrinsic Remediation: Principles, Risk Assessment And The Regulators
Mike Francis

Chapter 4: Gene Transfer (Plants)

Gene Transfer Between Canola (Brassica Napus) And Related Weed Species
Jack Brown, Donn C. Thill Angela P. Brown, Traci A. Brammer and Hari Nair

Gene Flow From Cultivated To Wild Strawberry (Fragaria Spp.)
K. Sossey-Alaoui, S. Rajapakse, M. B. Miller, A. G. Abbott, D. W. Tonkyn and T. P. Spira

RNA Recombination In Virus Resistant Transgenic Plants
Richard F. Allison, Ann E. Greene, and William L. Schneider

Potential Gene Flow Between Cultivated Potato And Its Wild Tuber-Bearing Relatives: Implications For Risk Assessment Of Transgenic Potatoes
S. A. Jackson and R.E. Hanneman, Jr.

Assessing The Effectiveness Of Border Areas In Confining The Spread Of Transgenic Brassica Napus Pollen
B.K. Staniland, P.B.E. McVetty, L.F. Friesen, S. Yarrow, P. Thiel, G. Freyssinet, and M. Freyssinet

Chapter 5: Gene Stability (Plants)

Increased Fitness Of Transgenic Insecticidal Canola Under Insect Selection Pressure
C. Neal Stewart, Jr., John N. All, Paul L. Raymer, and Suresh Ramachandran

Fitness Of F1 Wild-Crop Hybrids In Sunflower: A Progress Report
Allison A. Snow, Pedro Moran-Palma, Loren H. Rieseberg, and Gerald J. Seiler

Chapter 6: Field/Risk Assessment (Plants)

Assessment And Development Of Policy On Releases Of Genetically Modified Plants
Firoz Amijee

Risk Assessment For The Release Of Transgenic Trees: Should It Be Different Than With Crop Plants?
Charest, P.J., A. Seguin and A. Plourde

An Engo Perspective On The Biotechnology Risk Assessment Debate
Mark Winfield

Chapter 7: Gene Transfer and Stability (Microorganisms)

Recombination Between Cauliflower Mosaic Virus And Transgenic Plants Under Conditions Of Strong And Moderate Selection Pressure
James E. Schoelz, and William M. Wintermantel

TN916 Mediated Genetic Exchange In Soil
Bradley J. Haack, Robert E. Andrews Jr., and Thomas E. Loynachan

Transgenic Resistance To Barley Yellow Dwarf Virus: Replication, Recombination, And Risks
Gennadiy Koev, B.R. Mohan, Randy Beckett, and W.Allen Miller

Recombination Of A Transgene Transcript With Both The Plus Strand And The Minus Strand Viral RNA In Plants
Zhongguo Xiong and Ziming Weng

Sequence Mutability Of Clonal Viral Populations During Long-Term Passaging In Divergent Plant Hosts
C. M. Kearney, M. Thomson, K. E. Roland, and W.O. Dawson

Chapter 8: Risk Assessment: (Microorganisms)

Risk Assessment Protocols For Microbes
David Malloch and Iftikhar Ahmad

Environmental Risk Assessment Of Microbial Pest Control Products In Canada
Ted Kuchnicki and Brian Belliveau

The Risk Of Risking Sustainable Ecological Restoration
Kalin, M.

Chapter 9: Monitoring

Quantitative Detection Of Pentachlorophenol-Degrading Pseudomonas Sp. UG30 In Soil By A Most-Probable-Number/Polymerase Chain Reaction Protocol
K.T. Leung, A. Watt, H. Lee, and J.T. Trevors

Identification Of Plant Viral Synergism Genes
Vicki Bowman Vance

Use Of Strain-Specific Monoclonal Antibodies To Quantify Within-Field Temporal And Spatial Spread Of Soybean Mosaic Virus In Soybean
Forrest W. Nutter, Jr., Patricia M. Schultz, and John H. Hill

Capability Of Recombinant Insect Viruses For Environmental Persistence/Transport
J. R. Fuxa, S. A. Alaniz, A. R. Richter, L. M. Reilly, and B. D. Hammock

Microcosms For Evaluating Survival Of Pseudomonas Chloroaphis 3732RN-L11 In Soil
J. Angle, M. Levin, J. Gagliardi, C. Chanway, J. Germida, R. Watson, C. Wyndham, H. Yu, T. Mcintyre

Chapter 10: Fish

Triploids For Biological Containment: The Risk Of Heteroploid Mosaics
Standish K. Allen, Jr. and Ximing Guo

Use Of Multigenerational Studies To Assess Genetic Stability, Fitness, And Competitive Ability Of Transgenic Japanese Medaka: III. Results And Predictions
William M. Muir, Richard D. Howard, Robert S. Martens, S. Schulte, and Christopher A. Bidwell

Environmental Risks In Using GH Transgenic Atlantic Salmon And Rainbow Trout For Commercial Marine Production In Canada
Arnold Sutterlin, Garth Fletcher, Choy Hew, and Tillmann Benfey

Chapter 11: Posters

Tracking Transgenic Plants Using Green Fluorescent Protein
Staci Leffel, Stephen A. Mabon, and C. Neal Stewart, Jr.

Biosafety Guidance For Conducting Experimental Field Studies With Microorganisms
T. Edge, M.A. Levin, J.S. Angle, M.A. Gealt

Accumulation Of Heavy Metals By A Bacterium Isolated From Electroplating Effluent
F. Malekzadeh, A. Farazmand, H. Ghafourian, M. Shahamat, M. Levin, C. Grim, and R.R. Colwell

Marker Rescue Transformation Of Bacillus Subtilis Using The Transgenic Plant Marker Gene NPT-11: Preliminary Results
Franco Widmer, Kathleen Duncan, and Ramon J. Seidler

Comparison Of Rhizosphere Microbial Communities Of Parental And Transgenic Alfalfa By Community-Level Metabolic (BIOLOG) And Molecular (ERIC-PCR) Analyses
G.D. Di Giovanni, L.S. Watrud, F. Widmer, W.G. Landis, G. Mobus, and R.J. Seidler

Appendix A: Agenda of 1996 Symposium

Agenda Of 1996 Biotechnology Risk Assessment Symposium