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Proceedings and Papers
from the 1995 Risk Assessment Research Symposium


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Applications for Risk Assessment
Chapter 1. Gene Stability, Transfer and Expression
Chapter 2. Development of Risk Assessment Models
Chapter 3. Environmental Effects of Transgenic Organisms
Chapter 4. Assessing Effects of Field Trials
Chapter 5. Risk Assessment Strategies
Chapter 6. Monitoring Transgenic Organisms
Chapter 7. Posters Presented
Appendix A. Agenda

Applications for Risk Assessment

The Utility Of Bioassays In The Risk Assessment Of Entomopathogenic Fungi
Mark S. Goettel

Opportunities And Constraints For Bioremediation Of Soils: The Mexican Case
José Luis Solleiro, Rosario Castañón and Rodolfo Quintero

Chapter 1. Gene Stability, Transfer and Expression

Genetic Engineering Of Tree Species: The Canadian ExperiencE
Pierre J. Charest

Stability Of Disabled Viroid Sequences In Transgenic Plants: Generation Of Wild-Type Viroids Via Sequence Reversion And Recombination
Robert A. Owens, Rosemarie W. Hammond, and T.O. Diener

Does Expression Of A Defense Transgene Alter Nontarget Rhizosphere Microflora?
R.M. Goodman, L. Jacobson, B. Quirino,J. Handelsman, and D.A. Joseph

Growth And The Potential For Conjugation By Bacillus SphaericusIn Mosquito Larval Cadavers
Margarita Correa and Allan A.Yousten

Gene Transfer Between Canola (Brassica Napus L.) And Related Weed Species
J. Brown, D.C. Thill, A.P. Brown, C. Mallory-Smith, T.A. Brammer and H.S. Nair

Isolation Of Enterococcus Faecalis Exhibiting Conjugal Tetracycline From Swine Lot Outflow
Bradley J. Haack, Robert E. Andrews Jr., and Thomas E. Loynachan

Chapter 2. Development of Risk Assessment Models

The Patch Dynamics Of Horizontal Gene Transfer And Implications For The Risk Assessment Of Plasmids
Wayne G. Landis and Julann A. Spromberg

Model For Dispersal And Epiphytic Survival Of Bacteria Applied To Crop Foliage
Guy R. Knudsen and Louise-Marie C. Dandurand

Risk Of Alfalfa Transgene Dissemination And Scale Dependent Effects
Paul C. St. Amand, Daniel Z. Skinner, and Richard N. Peaden

Predator Avoidance, Spawning And Foraging Ability Of Transgenic Channel Catfish With Rainbow Trout Growth Hormone Gene
Rex A. Dunham, T.T. Chen, Dennis A. Powers, Amy Nichols, Brad Argue and C. Chitmanat

Use Of Multigenerational Studies To Assess Genetic Stability, Fitness, And Competitive Ability Of Transgenic Japanese Medaka: II. Production Of Transgenic Fish And Preliminary Studies
William M. Muir, Richard Howard, Robert Martens, and Christopher A. Bidwell

Chapter 3. Environmental Effects of Transgenic Organisms

Introduced Bacteria In Streams: The Effect Of Macroinvertebrates On Survival
Laura G. Leff and Anuradha Janakiraman

Identification Of Plant Viral Synergism Genes
Xing Ming Shi, Gail Pruss, Xin Ge, Verl Sriskanda and Vicki Bowman Vance

Rhizobium Meliloti Rhizopine Catabolism Genes: Distribution, Role In Competition And Potential As Marker Gene To Track Microbes
Silvia Rossbach, Brian McSpadden, Michelle Ganoff, and Frans J. de Bruijn

Persistence Of Npt-II Dna From Transgenic Plants In Soil: Laboratory And Field Observations
Franco Widmer, Ramon J. Seidler, and Lidia S. Watrud

Risk Assessment Of A Vaccinia Virus Double Recombinant Vaccine For Rinderpest
T. Yilma, L. Jones, F. Yoseph, and M.G. Yesus

Virulence Of Metarhizium Anisopliae To Embryos Of The Grass Shrimp Palaemonetes Pugio
Fred J. Genthner, Steven S. Foss and Patricia S. Glas

Construction And Characterization Of Pseudomonas Aeruginosa ATN Mutants
S. Elizabeth George, Xin Zhou and Paul S. Cohen

Current Research Priorities Pertaining To Health Requirements For Biotechnology Products Under The Canadian Environmental Protection Act
A. Godfrey, M. Campbell, J.Lam, W. Paranchych, D. Speert, and D.E. Woods

Chapter 4. Assessing Effects of Field Trials

Risk Assessment Of Virus Spread Using Transgenic Plants Containing Viral Coat Protein Genes
Marc Fuchs, Ferdinand Klas and Dennis Gonsalves

Examination Of The Environmental Impact Of A Genetically-Modified Plant Growth Promoting Rhizobacterium
Walter F. Mahaffee, Ellen M. Bauske, Joseph J. Shaw, and Joseph W. Kloepper

Assessing The Fate And Effects Of Microorganisms Under The Canadian Environmental Protection Act
Tom Edge

Risk Associated With Transgenic Resistance To Barley Yellow Dwarf Viruses
B.R. Mohan, G. Koev, and W.A. Miller

Sexual Cycle And Potential For Gene Flow In Fungal Biological Control Agents
Cindy R. Cisar and David O. TeBeest

Chapter 5. Risk Assessment Strategies

Genome Analysis Of Burkholderia Cepacia AC1100
William Hendrickson, Anette Hübner, Andrew Kavanaugh-Black and Reva Edelstein

Chromosomal Multiplicity In Burkholderia Cepacia
Thomas G. Lessiea and Brendan D. Manning

Assessing The Influences Of Predation And Resource Limitation On Microbial Populations
Paul W. Lepp and Thomas M. Schmidt

Cost Of Transgenic And Nontransgenic Herbicide Resistance Genes In Arabidopsis Thaliana
Colin B. Purrington and Joy Bergelson

The Impact Of Habitat Patchiness And Bacterial Genotype On Spread Of Bacteria In The Phyllosphere
Susan S. Hirano, Brendan K. Riely, Keith D. Fourrier, L. Stuart Baker, and Christen D. Upper

Chapter 6. Monitoring Transgenic Organisms

Aquatic Effects Monitoring In The Mining Industry: Review Of Appropriate Technologies
Danielle Rodrigue, Karen Mailhiot, Thomas P. Hynes, Linda J. Wilson and Marcia Blanchette

Predicting The Fate Of Engineered Baculoviruses
John Burand, Greg Dwyer, and Joseph S. Elkinton

Real-Time Monitoring Of MPCA Hazards To Honey Bees In A Microbial Containment Flight Chamber
J.J. Bromenshenk, B. Lighthart, R.L. McGraw II, M. R. Loeser, B. Birch, and P.J. Bowman

Chapter 7. Posters Presented

Predicting The Spread Of Insect Resistance: A Tool To Evaluate Ecological Risk
Joy Bergelson and Juliette Winterer

Pilot Studies Comparing Above And Below-Ground Effects Of Radionuclide Accumulation In Selected Species
James A. Entry, Lidia S. Watrud, Paul Rygiewicz, and William H. Emmingham

Potential Risks Of Transgenic Arthropod Biological Control Agents
Marjorie A. Hoy

Some Aspects Of The Environmental Safety Of Commercial Oil Spill Bioremediation Agents
Jeffrey L. Kavanaugh, C. Richard Cripe, Carol B. Daniels, Wallace T. Gilliam, Rochelle Araujo and Joe E. Lepo

Characterization Of Nitrogen-Fixing Bacterial Rhizosphere Communities Using Restriction Fragment Length Polymorphisms Of PCR- Amplified NIFH
Joe E. Lepo, Marisa K. Chelius, and David E. Weber

Comparing Potential Health Effects Of Intra-Nasal And Peroral Exposure Of Mice To Pseudomonas Aeruginosa And Escherichia Coli
Gail M. Nelson, S. Elizabeth George, Michael J. Kohan, and Jørgen Schlundt

Conjugal Gene Transfer In The Rhizosphere Of Water Grass (Echinochlora Crusgalli): Influence Of Root Exudate And Bacterial Activity
N. Kroer, T. Barkay, S.J. Sørensen and D. Weber

Factors Controlling The Population Dynamics Of Introduced Bacteria In Streams: A Tiered Approach To Their Study
Thomas L. Bott and Louis A. Kaplan

Phenotypic Conversions As A Result Of Pseudolysogeny
Julie J. Shaffer, John O. Schrader, and Tyler A. Kokjohn

Appendix A. Agenda

7Th Symposium On Environmental-Releases Of Biotechnology Products: Risk Assessment Methods And Research Progress