APPENDIX A

7TH SYMPOSIUM ON ENVIRONMENTAL-RELEASES OF BIOTECHNOLOGY PRODUCTS: RISK ASSESSMENT METHODS AND RESEARCH PROGRESS

June 6-8, Pensacola, Flo1rida

Tuesday, June 6

8:00 Registration and Poster Set-up

8:30 Introduction and Charge to Meeting: Dr. Robert E. Menzer, U.S. EPA, Dr.

David MacKenzie, USDA/CSRS, Dr. Terry McIntyre, Environment Canada

9:00 Dr. Mark Schaefer, Office of Science and Technology Policy, the White

House, "New Developments in Risk Assessment"

9:30 Dr. Gary Sayler, University of Tennessee, "Towards Field Release of

Engineered Strains for Bioremediation"

10:00 Dr. Mark Goettel, Agriculture and Agri-Food Canada, "The Utility of

Bioassays in the Risk Assessment of Entomopathogenic Fungi"

10:30 Dr. Jose Luis Solleiro, Visiting Research Fellow, International

Development Research Center, "Opportunities and Constraints to the

Enhanced Use of Bioremediation in Mexico"

11:00 LUNCH

RESEARCH SESSION 1: GENE STABILITY, POTENTIAL FOR GENE TRANSFER AND UNINTENDED EXPRESSION. SESSION CHAIR: James Harvey, U.S. EPA

12:00 R.B. Miller, Environmental Factors Affecting Bacterial Virus-Host

Interactions

12:20 P. Charest, Genetic Engineering of Forest Tree Species - The Canadian Experience

12:40 R.A. Owens, R.W. Hammond, and T.O. Diener, Stability of Disabled Viroid Sequences in Transgenic Plants: Generation of Wild-Type Viroids via Sequence Reversion and Recombination

1:00 R.M. Goodman, L. Jacobson, B. Quirino, J. Handelsman, and D. Joseph. Does Expression of a Defense Transgene Alter Nontarget Rhizosphere Microflora

1:20 A. Yousten and M. Correa, Potential for Growth and Gene Transfer by the Mosquito Pathogen, Bacillus sphaericus

1:40 I. Hwang and S.K. Farrand, Assessing Heterologous Horizontal Gene Transfer Between A Soil Organism and Genetically Modified Microorganisms

2:00 J. Brown, C. Mallory-Smith and D.C. Thill, Gene Transfer Between Canola (Brassica napus L) and Related Weed Species

2:20 B.J. Haack. R.E. Andrews, Jr., and T.E. Loynachan, Isolation of Enterococcus faecalis Exhibiting Conjugal Tetracycline Resistance from Swine Lot Outflow

RESEARCH SESSION 2: MODEL DEVELOPMENT. SESSION CHAIR: Dr. T. McIntyre, Environment Canada

2:40 W G. Landis, J.A. Spromberg, G. Mobus, C. Pickerign, G.B. Matthews, Non-Equilibrium Models, the Patch Dynamics of Horizontal Gene Transfer and Competitive Interactions and Implications for the Risk Assessment of Biotechnology-Based Products.

3:00 G. Knudsen and L. Dandurand, Model for Dispersal and Epiphytic Survival of Bacteria Applied to Crop Foliage

3:20 D.Z. Skinner, P. St. Amand and R.N. Peaden, Risk of Alfalfa Transgene Dissemination and Scale Dependent Effects

3:40 R. Dunham, Predator Avoidance, Spawning and Foraging Ability of Transgenic Catfish

4:00 W. Muir, R. Howard, R. Martens and C. Bidwell, Use of Multigenerational Studies to Assess Genetic Stability, Fitness, and Competitive Ability of Transgenic Japanese Medaka: II. Production of Transgenic Fish and Preliminary Studies

4:20 L. Cifuentes, Influence of Nutrient Loading Ratios on Algal Production in Estuaries: Implications for Nutrient Enrichment during Bioremediation.

4:40 ADJOURN

5:00 Laboratory Tour

Wednesday, June 7

RESEARCH SESSION 3: EFFECTS. SESSION CHAIR: Dr. L Watrud, U.S. EPA

8:00 W. Jeffrey, and David L Mitchell, Ultraviolet Radiation Induced Stress in Marine Bacterioplankton

8:20 L. Leff, Introduced Bacteria in Streams: The Effect of Macroinvertebrates on Survival and Gene Transfer

8:40 V. Vance, J. Carrington, and X. Shi, Identification of Plant Viral Synergism Genes

9:00 S. Rossbach, B. McSpadden, M. Ganoff and F.J. deBruijn, Rhizopine Synthesis and Catabolism Genes to Monitor Soil Bacteria and to Create 'Biased Rhizospheres'

9:20 K. Donegan, D. Schaller, V. Fieland, C. Palm, and R. Seidler, Decomposition of Genetically-Engineered Tobacco under Field Conditions: Persistence of the Proteinase Inhibitor I Product and Effects on Soil Organisms

9:40 F. Widmer, R. Seidler and L. Watrud, Persistence of NPT-II DNA from Transgenic Plants in Soil: Laboratory and Field Observations

10:00 T. Yilma, L. Jones, F. Yoseph and M.G. Yesus, Risk Assessment of a Vaccinia Virus Double Recombinant Vaccine for Rinderpest

10:20 Fred Genthner, Steven S. Foss, and Patricia S. Glas, Infectivity of Metarhizium anisopliae in Grass Shrimp Embryos

10:40 H. Alan Wood, Potential Replication of Recombinant Baculoviruses in Non-target Insect Species: Reporter Gene Products as Indicators of Infection.

11:00 R.R. James, B.T. Shaffer, B.A. Croft, and B. Lighthart, Non-Target Effects of Beauveria bassiana on the Convergent Lady Beetle, Hippodamia convergens: A Comparison of Laboratory, Greenhouse and Field Experiments

11:20 S. Elizabeth George, and Paul S. Cohen, Construction and Characterization of Pseudomonas aeruginosa atn Mutants.

11:40 A. Godfrey, Current Research Priorities Pertaining to Health Requirements for Biotechnology Products Under the Canadian Environmental Protection Act.

12:00 LUNCH

RESEARCH SESSION 4: FIELD TRIALS/RISK ASSESSMENTS. SESSION CHAIR: Dr. S. Shantaram, USDA

1:00 N. Skipper, Underlying Principles of Risk Assessment of Microorganisms under the CEPA.

1:20 D.G. Heckel and L.H. Gahn, Genetic Risk Assessment of Tobacco Budworm Resistance to Transgenic BT Toxin

1:40 M. Fuchs and D. Gonsalves, Risk Assessment of Virus Spread Using Transgenic Plants Containing Viral Coat Protein Genes

2:00 W.F. Mahaffee, E.M. Buske, J. Shaw and J.W. Kloepper, Examination of the Environmental Impact of Genetically-Modified Plant Growth Promoting Rhizobacterium

2:20 R.M. Goodman, S. Bintrim, M. Clayton, T. Donohue, J. Handelsman, D. Joseph, B. Quirino, and G. Roberts, New Approaches to Microbial Community Analysis in Soil and Rhizosphere

2:40 A. Matin, L. Watrud, Y-J. Kim, The Starvation Promoters of Pseudomonas: Regulation and Practical Applications.

3:00 S. Knudsen and P. Tsingelis, A Bacterium that Depends on Lactose for Repression of Suicide.

3:20 Snyder, R.A., M. James Hendry, and John R. Lawrence, Release of Burkholderia cepacia G4 PR1 for Aquifer Bioremediation: Analysis of Physical and Biological Factors Affecting Transport and Survival

3:40 T. Edge, Assessing the Fate and Effects of Microorganisms under the Canadian Environmental Protection Act.

4:00 B.R. Mohan, G. Kaev and W.A. Miller, Risks Associated with Transgenic Resistance to Barley Yellow Dwarf Viruses

4:20 R.E. Hanneman, Jr., and S.A. Jackson, Studies on the Crossability Relationships Between Commercial Varieties and Wild Species of Potato

4:40 D.O. Tebeest and C.R. Cisar, Sexual Cycle and Potential for Gene Flow in Fungal Biological Control Agents

4:40-5:30 POSTER PRESENTATION VIEWING

J. Bergelson, and J. Winterer, Predicting the Spread of Insect Resistance: A Tool to Evaluate Ecological Risk

J.A. Entry, P.T. Rygiewicz, and L.S. Watrud, Pilot Studies Comparing Above and Below Ground Ecological Effects of Radionuclide Accumulation in Selected Plant Species.

M. Hoy, Impact of Risk Analyses on Pest-Management Programs Employing Transgenic Arthropods.

J.L. Kavanaugh, C.R. Cripe, C.B. Daniels, R. Araujo, and J.E. Lepo, The Environmental Safety of Commercial Oil Spill Bioremediation Agents

J.E. Lepo, M.K. Chelius and D.E. Weber, Characterization of Nitrogen-fixing Bacterial Rhizosphere Communities Using Restriction Fragment Length Polymorphisms of PCR-Amplified nif H.

G. Mobus, C. Pickerign, M. Roze, M. DeMann, G.B. Matthews, and W.G. Landis, Artificial Intelligence Based Tools for the Analysis of Complex and Dynamic Patterns in the Risk Assessment of Biotechnology-Based Products.

G.M. Nelson, S. Elizabeth George, Michael J. Kohan, Jorgen Schlundt, Comparing Potential Health Effects of Intranasal and Peroral Exposure of Mice to Pseudomonas aeruginosa and Escherichia coli.

W. Pfender, S. Maggard, and L Watrud, Comparison of Three Bioremediation Field Agents for Metabolism of PCP and Use of Bioassays to Measure Toxicity In Bench-Scale and Field Bioremediation.

L.A. Porteous, F. Widmer, R. Seidler and L. Watrud, An Improved Method to Purify DNA Extracted from Environmental Samples or Quantitative Spectrophotometric Analysis and Applications to DNA Fingerprinting

K.R. Rogers and F.S. Ligler, Fiber Optic Biosensor for Detection of Bacterial Transport in Saturated Geologic Media

Thomas L. Bott and Louis A. Kaplan, Factors Controlling the Population Dynamics

of Introduced Bacteria in Streams: a Tiered Approach to Their Study

Julie J. Shaffer, John O. Schrader, and Tyler A. Kokjohn, Phenotypic Conversions as a Result of Pseudolysogeny

Thursday, June 8

RESEARCH SESSION 5: METHOD DEVELOPMENT AND APPLICATION. SESSION CHAIR: Dr. M. Levin, University of Maryland

8:00 G. Zylstra, Evolution of Catabolic Operons in P. cepacia

8:20 W. Hendrickson, Genetic and Physical Analysis of the Burkholderia cepacia AC1100 Genome

8:40 T. Lessie, Chromosomal Multiplicity in Pseudomonas cepacia.

9:00 R. Devereux, and S.G. Willis, Phylogeny of Some PAH-Degraders and Group II Pseudomonads Inferred from 16S rRNA Sequence Comparison

9:20 M. Hines, Molecular Phylogenetic Studies of the Structure and Function of Rhizosphere Bacterial Communities.

9:40 T. Schmidt, Unraveling the Diversity of Picoplankton and Controls on the Population Size Using Ribosomal RNAs

10:00 D.L. Santavy, T. Schmidt, and S.S. Wilkinson, Phylogeny of Phormidium corallyticum Using 16S rRNA

10:20 B.K. Passmore, T. Erikson, B.W. Falk, and G. Bruening, Recombination between Mutants of Beet Western Yellows Luteovirus and Between Mutants of Its Associated Subviral RNA Replicon

10:40 J.M. Bergelson and C. Purrington, Cost of Transgenic and Nontransgenic Herbicide Resistance genes in Arabidopsis thaliana

11:00 A. Snow and L.H. Reisenberg, Fitness of Wild-Crop Hybrids of Sunflower (Helianthus annuus)

11:20 S.S. Hirano, B.K. Riely, K.D. Fourrier, L.S. Baker and C.D. Upper, The Impact of Patchiness and Life Strategies on Spread of Phyllosphere Bacteria

11:40 LUNCH

RESEARCH SESSION 6: MONITORING. SESSION CHAIR: Hans Yu, Agriculture and Agri-Food Canada

1:00 J.E. Loper, S.E. Lindow, J. Kraus, M.D. Henkels, and C. Hopkins. Biological Sensors for Assessment of Microbial Activity and Chemical Constituents of Natural Habitats.

1:20 M. Blanchette, Aquatic Effects Technology Evaluation Program at Natural Resources Canada - An Overview of Toxicity and Monitoring Initiatives

1:40 L Cifuentes, New Stable Isotope Approaches for Monitoring Bioremediation

2:00 R. Watson, Monitoring Rhizobia in the Environment by PCR

2:20 J.P. Burand, Predicting the Fate of Engineered Baculoviruses

2:40 J.J. Bromenshenk, B. Lighthart, K.C. Smith, R.L. McGraw II, B. Birch, and P.J. Bowman, Real-Time Monitoring of MPCA Hazards to Honey Bees in a Microbial Containment Flight Chamber.

SESSION 7: INTERAGENCY DISCUSSION: RISK ASSESSMENT RESEARCH PLANNING. SESSION CHAIRS: Dr. Robert E. Menzer, Dr. David MacKenzie, Dr. Terry McIntyre

3:00 RAPPORTEURS:

P. Sayre, Dr. W. Schneider USEPA

S. Shantaram, USDA

T. Edge, Environment Canada

Hans Yu, Agriculture and Agri-Food Canada

4:00 ADJOURN