A Practical Guide to Containment
Plant Biosafety in Research Greenhouses
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Introductory Information
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This Guide was originally published in 2001 as A Practical Guide to Containment: Greenhouse Research with Transgenic Plants and Microbes and primarily addressed containment of transgenic plants or plant-associated organisms.

The reader will find information on
  • Biosafety containment levels
  • Physical and biological strategies
  • Modifications to achieve containment
  • Greenhouse management
  • Handling of biological materials
  • Guidance for developing or renovating facilities
  • Strategies for research with exotics (non-native invasive species), pathogens, insects, GE plant-manufactured pharmaceuticals, and industrial compounds
  • High containment for quarantined organisms, including those included on the Select Agent list
We emphasize working closely with regulatory authorities when using this Guide to develop containment strategies for research greenhouses. Although we refer by default to agencies within the United States, we also welcome people residing outside the United States to use the Guide freely. It is our sincere desire that this updated Guide will be of even greater service to the research community.
Printed copies are available by completing a Request Form here. Check the box titled "A Practical Guide to Containment". The Guide is free of charge.
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